Apogee Flow Systems

www.apogeeflow.com

Innovative Flow Cytometers

ApogeeFlow was established in the year 2000 as a spinoff from a US Army/LANL environmental bacteria detection program. ApogeeFlow has developed into a global leader offering a range of flow cytometers, becoming a reference system for studies of extracellular vesicles, liposomes and viruses. Their range of flow cytometers starts with the compact flow cytometers for conventional applications scaling up to a new class of flow cytometer for small particle studies and sorting.

ApogeeFlow's "MicroPLUS" cytometer is optimised for small particle applications without sacrificing the speed (event rates up to 100,000 per second) required for the measurement of small subpopulations which may originate from a particular cell type. ApogeeFlow was the first flow cytometer manufacturer to offer dedicated models optimised for small particle applications with the power to resolve particles scattering as little as a 100nm silica test bead.

The latest options include a wide range of laser colours, GxP compliance, light scatter calibration methods and walk-away operation for the analysis of 96 well plates.

MicroPLUS and Universal model flow cytometers can be configured for use in cGMP, cGLP and IVD environments.

Kairos' cLDEP Particle Sorter

The first ever application of centripetal liquid dielectrophoretic (cLDEP) force to particle sorting: An electric field plucks chosen particles from the stream with Kairos. The method, invented by ApogeeFlow, requires no moving parts and yields far higher speed and precision than piezoelectric methods.

User defined regions of interest on the flow cytometer's histograms allow selection of populations of particles to be sorted based on combinations of any optical parameter. The sort decision is made within 10μs of the laser interrogation and an electric field generated at the moment the particle reaches the sort zone giving high purity, yield and sort frequency. The automated system allows batches of samples to be sorted from and to a 96 well microplate, unattended.

NEW: IOTA Nanoparticle Upgrade

Analyse sub-100nm particles and sort!

An IOTA upgrade offers the performance needed for sub-100nm particle analysis & sorting. New data acquisition electronics combined with optical and fluidic developments has unlocked opportunities to study VLPs, engineered nanoparticles, exosomes and nanovesicle drug delivery systems.